DNA Core Facility DNA Sequencing Genome Sequencing |
QC of nucleic acidsThe Agilent 2100 bioanalyzer enables scientist working with nucleic acids to carry out automated quality control, sizing and quantification. The system uses micro-fabrication technology to transfer samples onto miniature glass chips that contain a network of interconnected channels and reservoirs. Filling the channels with a gel matriix and the wells with buffer or sample allows electrophoresis to be carried out on a miniaturized scale. Sample componensts are detected by their fluorescence and translated into gel-like images (bands) and electropherograms (peaks). Investigators interested in bioanlysis of their samples should bring 5 ul total RNA @ 50-500 ng/ul in a 0.5 ml microcentrifuge tube. Up to 12 samples may be run at once, at a cost of $50/1-12 samples. |